Aspartokinase from Wheat Germ

Aspartokinase from wheat germ: isolation, characterization, and regulation.

Aspartokinase has been isolated from wheat germ and a preliminary survey made of its properties in a partially purified extract. The enzyme has an absolute requirement for ATP and a divalent metal ion. The phosphate donor can be either ATP or GTP, but other nucleotides are ineffective. Both magnesium and manganese will activate the enzyme, whereas calcium shows a trace amount of activity. The e...

Triphosphopyridine Nucleotide Diaphorase from Wheat Germ.

11. KREBS, H. A. and HENSELEIT, K. Untersuchungen iiber die harnstoffbildung im tierkorper. Zeit. physiol. Chem. 210: 33. 1932. 12. McELROY, W. D. and GLASS, H. B. Amino acid Metabolism. Pp. 300-320. Johns Hopkins Press, Baltimore 1955. 13. MUELLER, G. C., QUINN, E. M. and RUECKERT, R. R. The formation of 1-phosphoerythrulose-4-C" by homogenates of Swiss chard leaves. Arch. Biochem. Biophys. 55...

Wheat Germ Agglutinin

Procedures for the isolation, purification, and crystallization of wheat germ agglutinin are described. The agglutinin was purified 184-fold to homogeneity from commercial wheat germ lipase. A molecular weight of 23,500 was estimated for the protein by means of sedimentation equilibrium and sodium dodecyl sulfate gel electrophoresis. The agghxtinin is a glycoprotein. Amino acid and carbohydrate...

Wheat germ-derived Ceramides

aBatchSystem Using Wheat Germ Extract

modified and unnatural amino acids into protein are possible.t) However, the relatively small amount of product due to early stopping of the translational reaction has been the primary bottleneck for its application. To overcome this problcm, Spirin and co-workers developed a continuous-fiow cell-free translation system, called the CFCF system, in 1988.2) In this system, the protein synthesis i...